Advancement in medical technology has brought new ways of detecting heart diseases in the human body. This is through the use of cardiac Elisa kits. These are diagnostic tools that work with samples and reagents in determining the existence of problems in the heart. This is done through looking out for color change in the reagents.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
The device should also be stable. This is achieved through reducing the loss rate as much as may be possible. The tools should be stored in good conditions to ensure that they remain stable. Other environmental influences should be completely avoided. Appropriate environmental conditions should, therefore, be provided. These include; appropriate temperature, pressure and humidity. Somebody should be given the responsibility of controlling the temperature in the incubator at all times. Assigning one person to work on the experiment is also crucial in ensuring stability.
For this experiment to work, one needs to prepare all samples, standards and reagents. He/she should then add a small amount of the sample to every well and then incubate for about two hours. After this, he should aspire then add some reagent and again incubate for about an hour. After this, he must aspire the mixture and wash it three times. The next step is addition of substrate solutions and then incubating for another 20 to 25 minutes. The last step is addition of stop solution and making readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
Once the substrate solution has been added, no other part, except the wells, will contain Tropin I type 3. A color change will be exhibited in the reagents. Sulphuric acid is then added with the purpose of bringing the reaction to an end. The change in color is measured in terms of some special wavelengths.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
The device should also be stable. This is achieved through reducing the loss rate as much as may be possible. The tools should be stored in good conditions to ensure that they remain stable. Other environmental influences should be completely avoided. Appropriate environmental conditions should, therefore, be provided. These include; appropriate temperature, pressure and humidity. Somebody should be given the responsibility of controlling the temperature in the incubator at all times. Assigning one person to work on the experiment is also crucial in ensuring stability.
For this experiment to work, one needs to prepare all samples, standards and reagents. He/she should then add a small amount of the sample to every well and then incubate for about two hours. After this, he should aspire then add some reagent and again incubate for about an hour. After this, he must aspire the mixture and wash it three times. The next step is addition of substrate solutions and then incubating for another 20 to 25 minutes. The last step is addition of stop solution and making readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
Once the substrate solution has been added, no other part, except the wells, will contain Tropin I type 3. A color change will be exhibited in the reagents. Sulphuric acid is then added with the purpose of bringing the reaction to an end. The change in color is measured in terms of some special wavelengths.
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